产品简介：
Infliximab is a humanized antibody against tumor necrosis factor α (TNF-α) that is used in the treatment of Crohn’s disease and rheumatoid arthritis.
产品形式：蛋白原液 。每种装于冻存管中，单抗质量为标示数量1mg或者5mg，体积根据其浓度不同略有不同。
分子式：C6428H9912N1694O1987S46 分子量：149.1KD
质量标准：
Non-reduced CE-SDS:………………………………………….>90%;
SEC-HPLC:………………………………………………………….>95%;
Bacterial Endotoxins Test：…………………………………. .<2EU/ml
Residual Proteins of Host Cell: ……………………………. <100 ng/mg
Exogenous residual DNA: …………………………………. . <100pg/mg
Residual Protein A: ……………………………………………. .<100 ng/mg
Biological Activity:………………………………………….……. .80% to 125% of standard
储存条件：
-70 ± 15℃for long-term storage, 2-8℃for short-term storage, away from light.
运输条件：
干冰运输
使用建议：
meilunbio提供的产品为蛋白原液形式。每种装于冻存管中，单抗质量为标示数量1MG或者5MG，体积根据其浓度不同略有不同。
使用前可以根据自己实验需求进行稀释，溶剂建议用去离子无菌水(meilunbio货号MA0028，去离子无菌水(细胞培养用水))或者PBS（meilunbio货号MA0015 PBS(1X),细胞培养级）。
不建议使用含有防腐成分的稀释液进行稀释，会增加细胞或者动物毒性。
稀释前，计算加入多少体积溶剂，请参考购买批次质检单上蛋白浓度，或者询问我司，
举例，质检单显示该批次单抗蛋白原液蛋白浓度为 50mg/mL
对应5mg包装的蛋白原液体积为5mg（质量）/50mg/ml(浓度)=0.1mL，
实验需求蛋白浓度为10mg/ml, 需要稀释倍数为 50/10=5, 则需要加入0.4mL,即400ul PBS或者无菌去离子水即可。
若给药量为固定质量值，比如单次给药0.5MG，对应蛋白原液给药体积为10uL，建议稀释后，提高给药体积降低误差，因为注射器或者移液器吸头残留会影响实验效果。
稀释后的蛋白溶液，可于2-8度冷藏避光保存不超过2周，不建议长期储存。
避免反复冻融，每次冻融蛋白活性会降低约5%左右。
| 靶点 | TNF-α |
|---|---|
| 体外 | To some of the polyclonally or antigen-specific-stimulated cell cultures, 1, 10 or 100 lg/ml of infiximab (MSD, Whitehouse Station, NJ) was added at the time for T cell stimulation or 48 h after T cell stimulation. Consecutive cell culture supernatants were collected and stored every 24 h or at the end of the cell culture. The T cell proliferation was measured at the end of cell culture by pulsing the cells with 0.5 lCi of [3H] thymidine/well (Amersham, Arlington Heights) for the last 8 h, and the incorporated radioactivity was analysed using a scintillation counter. Reference：Dahlén R, et al. Infliximab inhibits activation and effector functions of peripheral blood T cells in vitro from patients with clinically active ulcerative colitis.Scand J Immunol. 2013 Sep;78(3):275-84. |
| 体内 | Infected AKR mice received 300 embryonated T. muris eggs in distilled water by oral gavage. A single injection of 5 mg/kg (paediatric dose) of infliximab , was given intraperitoneally (carrier 0·9% sodium chloride) 35 days post‐infection. Control antibodies were given at the same concentration; anti‐mouse TNF‐α , rat IgG1 or human IgG.hypercholesterolemic Ldlr-/- mice. Reference：B. M. Assas, et al. Anti‐inflammatory effects of infliximab in mice are independent of tumour necrosis factor α neutralization.Clin Exp Immunol. 2017 Feb; 187(2): 225–233. |
关键词: Infliximab 英夫利昔单抗