Eculizumab；依库珠单抗
分子量：148KD
简介：Eculizumab is a recombinant human IgG2/4κ monoclonal antibody used for the treatment of paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). Eculizumab acts as a terminal complement inhibitor that binds to the terminal complement component 5, or C5 and suppresses the complement cascade.
产品形式：蛋白原液。每种装于冻存管中，单抗质量为标示数量1MG或者5MG，体积根据其浓度不同略有不同。
质量标准：
Non-reduced CE-SDS:………………………………………….>90%;
SEC-HPLC:………………………………………………………….>95%;
Bacterial Endotoxins Test：…………………………………. .<10EU/ml
Residual Proteins of Host Cell: ……………………………. <100 ng/mg
Exogenous residual DNA: …………………………………. . <100pg/mg
Residual Protein A: ……………………………………………. .<100 ng/mg
Biological Activity:………………………………………….……. .80% to 125% of standard
储存条件：-70 ± 15℃for long-term storage, 2-8℃for short-term storage, away from light.
运输条件：干冰运输
使用建议：
meilunbio提供的产品为蛋白原液形式。每种装于冻存管中，单抗质量为标示数量1MG或者5MG，体积根据其浓度不同略有不同。
使用前可以根据自己实验需求进行稀释，溶剂建议用去离子无菌水(meilunbio货号MA0028，去离子无菌水(细胞培养用水))或者PBS（meilunbio货号MA0015PBS(1X),细胞培养级）。
不建议使用含有防腐成分的稀释液进行稀释，会增加细胞或者动物毒性。
稀释前，计算加入多少体积溶剂，请参考购买批次质检单上蛋白浓度，或者询问我司，
举例，质检单显示该批次单抗蛋白原液蛋白浓度为 50mg/ml
对应5MG包装的蛋白原液体积为5mg（质量）/50mg/ml(浓度)=0.1ML，
实验需求蛋白浓度为10mg/ml, 需要稀释倍数为 50/10=5, 则需要加入0.4ML,即400ul PBS或者无菌去离子水即可。
若给药量为固定质量值，比如单次给药0.5MG，对应蛋白原液给药体积为10ul，建议稀释后，提高给药体积降低误差，因为注射器或者移液器吸头残留会影响实验效果。
稀释后的蛋白溶液，可于2-8度冷藏避光保存不超过2周，不建议长期储存。
避免反复冻融，每次冻融蛋白活性会降低约5%左右。
生物活性（仅来自于公开文献，不保证其有效性）
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描述 |
Eculizumab is a recombinant human IgG2/4κ monoclonal antibody used for the treatment of paroxysmal nocturnal hemoglobinuria (PNH) and atypical hemolytic uremic syndrome (aHUS). Eculizumab acts as a terminal complement inhibitor that binds to the terminal complement component 5, or C5 and suppresses the complement cascade. |
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靶点 |
补体蛋白(C5) |
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Application |
the treatment of PNH and aHUS. |
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体内 |
A human FcRn (hFcRn) transgenic mouse strain was used to model the PK and PD of surrogate anti-mouse C5 antibodies with eculizumab-derived Fc domains. Preliminary studies showed that the chimeric murine-human antibodies elicited a high frequency of anti-drug antibodies in this mouse strain; therefore, animals were immune-suppressed with a murine anti-CD4 antibody administered in a 500 μg intraperitoneal loading dose at day -3 and weekly maintenance doses of 100 μg. Test articles were administered in a single 1000 μg IV dose on study day 0. Approximately 100 μL of blood was collected from the left retro-orbital sinus on study days -8, 1, 3, 7, 14, 21, 28, and 35. Serum was prepared and PK parameters were determined as described above. |
用途及描述：仅供科研，严禁用于人体(For R&D Only)
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