产品简介：
MEM培养基也称最低必需培养基，是在Eagle基本培养基（BEM）的基础上发展而来的，是一种最基本、应用最广的动物细胞培养基。MEM培养基仅含有12种必需氨基酸、谷氨酰胺和8种维生素，成分简单，主要用于贴壁细胞的培养，修改配方后的α-MEM则可广泛应用于各种哺乳动物悬浮和贴壁细胞培养。
MEM(Minimum Essential Medium) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of MEM modifications for a range of cell culture applications.
| With | Without |
|---|---|
| • NEAA | • HEPES |
| • L-glutamine | |
| • Phenol Red | |
| • D-Glucose (Dextrose)(1000mg/L) |
储存条件：
2-8°C
运输条件：
常温
Using MEM
MEM was developed by Harry Eagle, and was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for use without CO2. This product is made with Earle’s salts. MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
