重组人肝细胞生长因子(HGF)

¥1,110.00

规格: 10μg

英文名字:Hepatocyte Growth Factor,Human,Recombinant
质量标准:>95%,BR
货号: MA1351-1
Source: Human Cells
Description: Recombinant Human Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln32-Ser728 is expressed with a 6His tag at the C-terminus.
Accession: P14210
Known As: Hepatocyte growth factor; HPTA; HGF; SF; Scatter factor; Hepatopoietin-A
Predicted Mol Mass: 26&53.7 Kda
Apparent Mol Mass: 32-38&50-65 KDa, reducing conditions
Endotoxin: < 0.01 EU/µg as determined by LAL test.
Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris,500mM NaCl,3%Trehalose,0.02% Tween 80,pH 8.0.
Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Shipping: The product is shipped at ambient temperature.
Upon receipt, store it immediately at the temperature listed below.
Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Background: HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases and contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha and 30 kDa beta chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. It can also alter epithelium morphology by the induction of nectin-1 alpha ectodomain shedding, an adhesion protein component of adherens junctions. HGF regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto oncogenic c-Met receptor. HGF is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration.

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