L-Buthionine-sulfoximine(BSO)

L-Buthionine-sulfoximine(LBSO);
分子式：C8H18N2O3S
分子量：222.31
外观：white or off-white solid
溶解性：Water 50 mg/ml,
储存条件：−20°C
生物活性：Buthionine sulfoximine (BSO) is an irreversible inhibitor of γ-glutamylcysteine synthetase (Ki <100 μM), the rate-limiting enzyme for L-glutathione (GSH) synthesis, that induces oxidative stress in cells by depleting GSH. Administration of BSO leads to decreased GSH levels in virtually all tissues and is associated with tissue damage and apoptosis. Whereas elevated glutathione levels are associated with tumor cell resistance, BSO has been shown to enhance the toxicity of various chemotherapeutic agents in drug-resistant tumors

实验操作（仅供参考）
The Effect of L-Buthionine Sulfoximine on the Toxicities and Interactions of As, Cd, Hg and Pb and their Composite Mixture on MCF 7 Cell Line
(British Journal of Applied Science & Technology 5(5): 510-519, 2015, Article no.BJAST.2015.049)
Materials and method
MCF 7 cells were grown in MEM alpha 1x supplemented with 10.0% FBS and 1.0% penicillin streptomycin and incubated for 24 hrs at 37°C in a 5% CO2 incubator to allow the cells to grow, and form a monolayer in the flask.
Cells grown to 75-85% confluence were washed with phosphate buffer saline (PBS), trypsinized with 3 mL of 0.25% (v) trypsin, 0.3% (v) EDTA, diluted with fresh medium, and counted for experimental purposes.

To inhibit the production of cellular GSH, growth medium containing 2.5mMLBSOwas used to seed MCF 7 cells in sterile 96-well (1 x 104 cells/well) plates and placed in a CO2 incubator for 24 hours. The concentration of LBSO used was pre-determined by exposing MCF 7 cells to decreasing concentrations of LBSO (starting form 20mM). The concentration that could effectively inhibit GSH production with a cell survival rate of 95% was determined to be 2.5mM.
Subsequently cells were exposed to serially diluted concentrations of the individual metals and the composite mixture of the 4 metals prepared in MEM (without phenol), supplemented with 5% penicillin streptomycin. The highest concentration for the individual chemicals was 80 mg/L. The mixture of the four metals was made by mixing As, Cd, Hg and Pb stock solutions in ratio of their EPA’s MCL 10:5:2:15 respectively representing a starting concentration of 20, 10, 4 and 30 mg/L respectively. The first row of each plate was used as control (medium without cell) and the second row used as negative control (cells without chemicals) and the treated cells were incubated for 24 hours.

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