Dasabuvir(ABT-333)
分子式：C26H27N3O5S分子量：493.57
简介：Dasabuvir(ABT-333) is a non-nucleoside inhibitor ofNS5B viral RNA-dependent RNA polymerasethat inhibits recombinant NS5B polymerases derived from HCV genotype 1a and 1b clinical isolates with IC50 values between 2.2 and 10.7 nM. It is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases.
物理性状及指标：
外观：……………………粉末
溶解性：…………………H2O : Insoluble ；DMSO：98 mg/mL (198.55 mM)；Ethanol：Insoluble
含量：……………………>98%
储存温度：-20℃，避光防潮密闭干燥
生物活性
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描述 |
Dasabuvir (ABT-333) 是一种非核苷抑制剂，抑制RNA依赖性的HCV NS5B基因编码的 RNA 聚合酶，抑制 HCV 基因型 1a 和 1b 临床分离株衍生的重组 NS5B 聚合酶，IC50为 2.2 到 10.7 nM。 | |||
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IC50& Target |
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体外 |
Dasabuvir (ABT-333) is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases. Dasabuvir (ABT-333) inhibits the polymerase enzymatic activity of genotype 1 laboratory strain enzymes (H77, BK, N, and Con1 strains), as well as enzymes produced from polymerase genes from HCV genotype 1-infected subjects, with IC50s between 2.2 and 10.7 nM. Dasabuvir (ABT-333) inhibits replication of HCV subgenomic replicons in cell culture assays, with EC50values of 7.7 and 1.8 nM against genotype 1a (H77) and 1b (Con1), respectively. In the presence of 40% human plasma, there is a 12- to 13-fold decrease in inhibitory potency, yielding EC50s of 99 and 21 nM for HCV genotype 1a (H77) and 1b (Con1) replicons, respectively. |
储液配置及储存：按表中溶解性配置；如溶解困难，可以通过快速搅拌，超声或温和加热（在45-60°C下水浴）。液体稳定性报道的很少，建议现配现用，如需储存，建议：-20℃1-3月；-80℃ 3-6月。
| 浓度/体积/质量 |
1 mg |
5 mg |
10 mg |
| 1 mM | 2.0261 mL | 10.1303 mL | 20.2606 mL |
| 5 mM | 0.4052 mL | 2.0261 mL | 4.0521 mL |
| 10 mM | 0.2026 mL | 1.0130 mL | 2.0261 mL |
| 50 mM | 0.0405 mL | 0.2026 mL | 0.4052 mL |
【注意】
●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。