XTT钠盐;XTT

产品简介：

XTT is used to assess cell viability as a function of redox potential. Actively respiring cells convert the water-soluble XTT to a water-soluble, orange colored formazan product. Unlike MTT, XTT does not require solubilization prior to quantitation, thereby reducing the assay time in many viability assay protocols. Moreover, the sensitivity of the XTT reduction assay is reported to be similar to or better than that of the MTT reduction assay.

化学名：2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide
分子式：C₂₂H₁₆N₇NaO₁₃S₂分子量：673.52
用途：用于细胞活力检测，灵敏度高于MTT

储存条件：

2-8°C

运输条件：

常温运输

使用方法（仅供参考）

细胞活性检测

XTT is a colorimetric assay used to assess cell viability as a function of cell number based on metabolic activity. This rapid, sensitive, non-radioactive assay is detected using standard microplate absorbance readers.

1. Grow cells in a 96-well plate at a density of 10⁴–10⁵ cells/well in 100 µL of culture medium with compounds to be tested. Culture in a CO₂ incubator for 24–48 hours
2. Make a 10 mM PMS solution in phosphate-buffered saline (3 mg PMS into 1 mL PBS)；PMS美仑货号MB6047
3. Dissolve 4 mg of XTT in 4 mL of 37°C cell culture medium
4. Add 10 µL of the PMS solution the 4 mL of XTT solution created in step 3immediatelybefore labeling cells
5. Add 25 µL of XTT/PMS solution directly to each well containing 100 µL cell culture
6. Incubate for 2 hours at 37°C in a CO₂ incubator
7. Read absorbance at 450 nm

关键词： XTT钠盐；XTT；