重组人TNF-β/TNFSF1（肿瘤坏死因子）；TNFβ/TNFSF1
简介：
TNFis secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF. TNF-β is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-β is produced by activated T and B lymphocytes, and has similar activities to TNF-α. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses.
RecombinantHuman TNF-βproduced inE. coliis is a glycosylated polypeptide chain of 172 amino acids. A fully biologically active molecule,rhTNF-β has a molecular mass of 18.6 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
别名：Tumor Necrosis Factor-β, human; TNF-beta, human,TNFSF1, Lymphotoxin-alpha (LT-α)
性状描述：
Species：……………………………Human
Source：………………………………E. coli
Measured Molecular Weight：……18.6kDa, observed by reducing SDS-PAGE.
Purity：………………………………> 95% as analyzed by SDS-PAGE.
Formulation：………………………Lyophilized after extensive dialysis against PBS.
Reconstitution：……………………Reconstituted in ddH2O or PBS at 100 μg/ml.
Sequence：
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK
QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV
VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK
MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL
SPSTVFFGAF AL
储存条件：Lyophilized recombinantHuman Tumor Necrosis Factor-beta (TNF-β)remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Human TNF-β should be stable up to 1 week at 4°C or up to 3 months at -20°C.For long term storage it is recommended that a carrier protein (example 0.1% BSA) can be added.Avoid repeated freeze-thaw cycles.
运输条件：2~8℃运输
生物活性：
ED50 < 4 pg/mL, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of >2.5 x 108 units/mg.
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用途及描述：科研试剂，广泛应用于分子生物学，药理学等科研方面，严禁用于人体。
【注意】
●我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。
●部分产品我司仅能提供部分信息，我司不保证所提供信息的权威性，以上数据仅供参考交流研究之用。